other:Article Title: SARS-CoV-2-Specific T Cells Exhibit Phenotypic Features of Helper Function, Lack of Terminal Differentiation, and High Proliferation Potential
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HLADR Thermofisher Cat#Q22158 RORgt Fisher Scientific Cat#5013565 CD49d (a4) Fluidigm Cat#3141004B CTLA4 Fisher Scientific Cat#5012919 NFAT Fluidigm Cat3143023A CCR5 Fluidigm Cat#3144007A CD20 Biolegend Cat#302343 CD95 Fisher Scientific Cat#MAB326100 CD7 Fluidigm Cat#3147006B ICOS Fluidigm Cat#3148019B Tbet Fisher Scientific Cat#5013190 IL4 Biolegend Cat#500829 CD2 Fluidigm Cat#3151003B IL17 Biolegend Cat#512331 CD62L Fluidigm Cat#3153004B TIGIT Fludigm Cat#3154016B CCR6 BD Biosciences Cat#559560 IL6 Biolegend Cat#501115 CD8 Biolegend Cat#301053 CD19 Biolegend Cat#302247 CD14 Biolegend Cat#301843 OX40 Fluidigm Cat#3158012B CCR7 Fluidigm Cat#3159003A CD28 Fluidigm Cat#3160003B CD45RO Biolegend Cat#304239 CD69 Fluidigm Cat#3162001B CRTH2 Fluidigm Cat#3163003B PD-1 Biolegend Cat#329941 CD127 Fluidigm Cat#3165008B CXCR5 BD Biosciences Cat#552032 CD27 Fluidigm Cat#3167006B IFNg Fluidigm Cat#3168005B CD45RA Fluidigm Cat#3169008B CD3 Fluidigm Cat#3170001B CD57 Biolegend Cat#359602 CD38 Fluidigm Cat#3172007B CD4 Fluidigm Cat#3174004B CXCR4 Fluidigm Cat#3175001B CD25 Biolegend Cat#356102 CD161 Biolegend Cat#339919 CD3 (APC/Cyanine) Biolegend Cat#344817 CD4 (PE/DazzleTM 594) Biolegend Cat#300547 CD8 (Alexa Fluor 700) Biolegend Cat#344723 (Continued on next page) Cell Reports Medicine 1, 100081, September 22, 2020 e1
Purification:Article Title: Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HLADR Thermofisher Cat#Q22158 RORgt Fisher Scientific Cat#5013565 CD49d (a4) Fluidigm Cat#3141004B CTLA4 Fisher Scientific Cat#5012919 NFAT Fluidigm Cat3143023A CCR5 Fluidigm Cat#3144007A IL5 Biolegend Cat#504309 CD95 Fisher Scientific Cat#MAB326100 CD7 Fluidigm Cat#3147006B ICOS Fluidigm Cat#3148019B Tbet Fisher Scientific Cat#5013190 IL4 Biolegend Cat#500829 CD2 Fluidigm Cat#3151003B IL17 Biolegend Cat#512331 CD62L Fluidigm Cat#3153004B TIGIT Fludigm Cat#3154016B CCR6 BD Biosciences Cat#559560 IL6 Biolegend Cat#501115 CD8 Biolegend Cat#301053 CD19 Biolegend Cat#302247 CD14 Biolegend Cat#301843 OX40 Fluidigm Cat#3158012B CCR7 Fluidigm Cat#3159003A CD28 Fluidigm Cat#3160003B CD45RO Biolegend Cat#304239 CD69 Fluidigm Cat#3162001B CRTH2 Fluidigm Cat#3163003B PD-1 Biolegend Cat#329941 CD127 Fluidigm Cat#3165008B CXCR5 BD Biosciences Cat#552032 CD27 Fluidigm Cat#3167006B IFNg Fluidigm Cat#3168005B CD45RA Fluidigm Cat#3169008B CD3 Fluidigm Cat#3170001B CD57 Biolegend Cat#359602 CD38 Fluidigm Cat#3172007B CD4 Fluidigm Cat3174004B CXCR4 Fluidigm Cat#3175001B CD25 Biolegend Cat#356102 CD161 Biolegend Cat#339919 Purified Mouse anti-Human CD49d BD Biosciences Cat#340976 Purified Mouse anti-Human CD28 BD Biosciences Cat#340975 (Continued on next page) e1 Cell Reports 36, 109414, July 20, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins LymphoprepTM STEMCELL Technologies Cat#07801 Cisplatin Sigma-Aldrich Cat#P4394 Paraformaldehyde Electron Microscopy Sciences Cat#15710 Metal Contaminant-Free PBS Rockland Cat#MB-008 Brefeldin A Solution Thermofisher Cat#00-4506-51 s PepMix SARS-CoV-2 Peptide (Spike Glycoprotein) JPT Peptide Technologies Cat#PM-WCPV-S PMA Sigma-Aldrich Cat#1585 Ionomycin calcium salt Sigma-Aldrich Cat#I0634 Normal Mouse Serum ThermoFisher Cat#10410 Normal Rat Serum ThermoFisher Cat#10710C Human Serum From Male AB Plasma Sigma-Aldrich Cat#H4511 Intracellular Fixation & Permeabilization Buffer ThermoFisher Cat#88-8823-88 Permeabilization Buffer ThermoFisher Cat#00-8333-56 Iridium Interchelator Solution Fluidigm Cat#201192B MaxPar cell staining buffer Fluidigm Cat#201068 Cell acquisition solution Fluidigm Cat#201240 EQ Four Element Calibration Beads Fluidigm Cat#201078 Critical commercial assays MaxPar X8 Antibody Labeling Kit Fluidigm Cat#201169B Cell-IDTM 20-Plex Pd Barcoding Kit Fluidigm Cat#201060 Deposited data Raw CyTOF datasets This paper http://datadryad.org/stash/dataset/doi:10.
|
fluidigm is a verified supplier 
fluidigm manufactures this product 